District 1 – Southwest Florida
Major Cities: Arcadia, Bartow, Fort Myers, Lakeland, Naples, North Port, Sarasota, Sebring, and Venice
Counties: Charlotte, Collier, De Soto, Glades, Hardee, Hendry, Highlands, Lee, Manatee, Okeechobee, Polk, and Sarasota
- Commute Connector
- Manatee County Area Transit (MCAT)
- Sarasota County Area Transit (SCAT)
- Lee County Transit (LeeTran)
- Citrus Connection (Lakeland)
- Polk County Transit Services (PCTS)
- Ride On Commuter Services
- StarMetro (Tallahassee transit service)
- Escambia County Transit
- Park and Ride Lots
- South Florida Commuter Services – commuter assistance
- Broward County Transit
- Tri-Rail
- PalmTran
- 95 Express
- Park and Ride lots
- reThink – District 5 Regional Commuter Assistance Program – ride matching, Emergency Ride Home, Employer Services
- Lynx (Central Florida Regional Transportation Authority) – transit, vanpools
- Space Coast Area Transit (SCAT) – transit, vanpools
- Volusia County Public Transportation (VOTRAN) – transit
- Sumter County Transit
- Ocala/Marion County Public Transportation System (SunTran)
- LakeXpress – transit
- Park and Ride Lots
- South Florida Commuter Services – commuter assistance
- Miami-Dade Transit
- Tri-Rail
- Hialeah Transit System
- City of Coral Gables Trolley
- 95 Express
- Park and Ride lots
- TBARTA Commute Tampa Bay – commuter & employer assistance
- New North Transportation Alliance – commuter & employer assistance
- St. Petersburg Downtown Partnership Transportation Management Organization (TMO) – The Looper trolley
- Tampa Downtown Partnership TMO – Commuter & employer assistance
- Westshore Alliance TMO – commuter & employer assistance
- Hillsborough Area Regional Transit (HART)
- Pinellas County Transportation
- Pasco County Public Transportation
- Clearwater Beach Jolley Trolley
- Park and Ride Lots